› 4xsk

X-ray diffraction
1.5Å resolution

Structure of PAItrap, an uPA mutant

Released:
DOI: 10.2210/pdb4xsk/pdb
PDB entry 4xsk coloured by chain, front view.
PDB entry 4xsk coloured by chain, side view.
PDB entry 4xsk coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Gong L, Proulle V, Hong Z, Lin Z, Liu M, Yuan C, Lin L, Furie B, Flaumenhaft R, Andreasen P, Furie B, Huang M

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 246 amino acids
Theoretical weight: 27.76 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P00749 (Residues: 179-424; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand PGE 2 x PGE
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 121.025Å b: 121.025Å c: 42.774Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.209
Expression system: Komagataella phaffii GS115

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