› 4xss

X-ray diffraction
3Å resolution

Insulin-like growth factor I in complex with site 1 of a hybrid insulin receptor / Type 1 insulin-like growth factor receptor

Released:
DOI: 10.2210/pdb4xss/pdb
PDB entry 4xss coloured by chain, front view.
PDB entry 4xss coloured by chain, side view.
PDB entry 4xss coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Congruency of Ligand Binding to the Insulin and Insulin/Type 1 Insulin-like Growth Factor Hybrid Receptors.
Menting JG, Lawrence CF, Kong GK, Margetts MB, Ward CW, Lawrence MC
Structure 23 1271-82 (2015)
PMID: 26027733

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero octadecamer
PDBe Complex ID:
PDB-CPX-138313 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Insulin-like growth factor I Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 7.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05019 (Residues: 49-118; Coverage: 40%)
Gene names: IBP1, IGF1
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 317 amino acids
Theoretical weight: 36.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains:
Structure domains:
Insulin-like growth factor 1 receptor alpha chain Chain: F
Molecule details ›
Chain: F
Length: 16 amino acids
Theoretical weight: 1.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P08069 (Residues: 721-736; Coverage: 1%)
Gene name: IGF1R

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 1 x SO4
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: R32
Unit cell:
a: 219.16Å b: 219.16Å c: 121.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.229
Expression systems:
  • Escherichia coli
  • Cricetulus griseus
  • Not provided

Quick links

Citations

9 review citations

Insulin Receptor Isoforms in Physiology and Disease: An Updated View.
Belfiore et al. (2017)
8 more

5 mentions without citation

How ligand binds to the type 1 insulin-like growth factor receptor.
Xu et al. (2018)
4 more