› 4xvj

X-ray diffraction
2Å resolution

STRUCTURE OF THE HEPATITIS C VIRUS ENVELOPE GLYCOPROTEIN E2 ANTIGENIC 2 REGION 412-423 BOUND TO THE BROADLY NEUTRALIZING ANTIBODY HC33.1

Released:
DOI: 10.2210/pdb4xvj/pdb
PDB entry 4xvj coloured by chain, front view.
PDB entry 4xvj coloured by chain, side view.
PDB entry 4xvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for penetration of the glycan shield of hepatitis C virus E2 glycoprotein by a broadly neutralizing human antibody.
Li Y, Pierce BG, Wang Q, Keck ZY, Fuerst TR, Foung SK, Mariuzza RA
J Biol Chem 290 10117-25 (2015)
PMID: 25737449

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210945 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein E2 Chain: A
Molecule details ›
Chain: A
Length: 13 amino acids
Theoretical weight: 1.55 KDa
UniProt:
  • Canonical: P27958 (Residues: 412-423; Coverage: 0%)
antibody heavy chain variable domain Chain: H
Molecule details ›
Chain: H
Length: 141 amino acids
Theoretical weight: 14.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody light chain variable domain Chain: L
Molecule details ›
Chain: L
Length: 115 amino acids
Theoretical weight: 11.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 36.085Å b: 53.728Å c: 107.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.274
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Genetic Diversity Underlying the Envelope Glycoproteins of Hepatitis C Virus: Structural and Functional Consequences and the Implications for Vaccine Design.
Tarr et al. (2015)
15 more

18 mentions without citation

Viral evasion and challenges of hepatitis C virus vaccine development.
Pierce et al. (2016)
17 more