› 4xx1

X-ray diffraction
3.6Å resolution

Low resolution structure of LCAT in complex with Fab1

Released:
DOI: 10.2210/pdb4xx1/pdb
PDB entry 4xx1 coloured by chain, front view.
PDB entry 4xx1 coloured by chain, side view.
PDB entry 4xx1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The high-resolution crystal structure of human LCAT.
Piper DE, Romanow WG, Gunawardane RN, Fordstrom P, Masterman S, Pan O, Thibault ST, Zhang R, Meininger D, Schwarz M, Wang Z, King C, Zhou M, Walker NP
J Lipid Res 56 1711-9 (2015)
PMID: 26195816

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229827 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab1 light chain Chains: G, L, O
Molecule details ›
Chains: G, L, O
Length: 213 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab1 heavy chain Chains: E, H, M
Molecule details ›
Chains: E, H, M
Length: 238 amino acids
Theoretical weight: 25.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Phosphatidylcholine-sterol acyltransferase Chains: A, B, J
Molecule details ›
Chains: A, B, J
Length: 422 amino acids
Theoretical weight: 47.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P04180 (Residues: 25-440; Coverage: 100%)
Gene name: LCAT
Sequence domains: Lecithin:cholesterol acyltransferase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand NAG 9 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P32
Unit cell:
a: 166.419Å b: 166.419Å c: 97.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.196 0.28
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

4 review citations

Molecular Pathways Underlying Cholesterol Homeostasis.
Afonso et al. (2018)
3 more

4 mentions without citation

The high-resolution crystal structure of human LCAT.
Piper et al. (2015)
3 more