› 4xxo

X-ray diffraction
2.84Å resolution

Crystal Structure of Human APOBEC3A

Released:
DOI: 10.2210/pdb4xxo/pdb
PDB entry 4xxo coloured by chain, front view.
PDB entry 4xxo coloured by chain, side view.
PDB entry 4xxo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The ssDNA Mutator APOBEC3A Is Regulated by Cooperative Dimerization.
Bohn MF, Shandilya SMD, Silvas TV, Nalivaika EA, Kouno T, Kelch BA, Ryder SP, Kurt-Yilmaz N, Somasundaran M, Schiffer CA
Structure 23 903-911 (2015)
PMID: 25914058

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3A Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31941 (Residues: 1-199; Coverage: 100%)
Gene name: APOBEC3A
Sequence domains: APOBEC-like C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand ZN 7 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 94.752Å b: 94.752Å c: 213.962Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

The APOBEC Protein Family: United by Structure, Divergent in Function.
Salter et al. (2016)
13 more

15 mentions without citation

A Novel Regulator of Activation-Induced Cytidine Deaminase/APOBECs in Immunity and Cancer: Schrödinger's CATalytic Pocket.
King et al. (2017)
14 more