› 4xz1

X-ray diffraction
2.8Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149098 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43403 (Residues: 1-259; Coverage: 42%)
Gene names: SRK, ZAP70
Sequence domains: SH2 domain
Structure domains:
T-cell surface glycoprotein CD3 zeta chain Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P20963 (Residues: 69-87; Coverage: 13%)
Gene names: CD247, CD3Z, T3Z, TCRZ
Sequence domains: Immunoreceptor tyrosine-based activation motif

Ligands and Environments

1 bound ligand:
Ligand 4N6 1 x 4N6
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 52.944Å b: 61.105Å c: 53.451Å
α: 90° β: 116.37° γ: 90°
R-values:
R R work R free
0.218 0.216 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

ZAP-70 in Signaling, Biology, and Disease.
Au-Yeung et al. (2018)
1 more

1 mention without citation

Computational Prediction of MoRFs, Short Disorder-to-order Transitioning Protein Binding Regions.
Katuwawala et al. (2019)