› 4xzf

X-ray diffraction
1.38Å resolution

Crystal structure of HIRAN domain of human HLTF in complex with DNA

Released:
DOI: 10.2210/pdb4xzf/pdb
PDB entry 4xzf coloured by chain, front view.
PDB entry 4xzf coloured by chain, side view.
PDB entry 4xzf coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a Novel DNA-binding Domain of Helicase-like Transcription Factor (HLTF) and Its Functional Implication in DNA Damage Tolerance.
Hishiki A, Hara K, Ikegaya Y, Yokoyama H, Shimizu T, Sato M, Hashimoto H
J Biol Chem 290 13215-23 (2015)
PMID: 25858588

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118297 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Helicase-like transcription factor Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14527 (Residues: 58-174; Coverage: 12%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1
Sequence domains: HIRAN domain
(DA)(DC)(DC)(DG)(DC)(DC)(DG)(DG)(DG)(DT)(DG)(DC)(DC) Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21212
Unit cell:
a: 147.399Å b: 33.418Å c: 36.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.207
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Replication Fork Reversal: Players and Guardians.
Quinet et al. (2017)
14 more

10 mentions without citation

Functions of SMARCAL1, ZRANB3, and HLTF in maintaining genome stability.
Poole et al. (2017)
9 more