› 4xzg

X-ray diffraction
2.4Å resolution

Crystal structure of HIRAN domain of human HLTF

Released:
DOI: 10.2210/pdb4xzg/pdb
PDB entry 4xzg coloured by chain, front view.
PDB entry 4xzg coloured by chain, side view.
PDB entry 4xzg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a Novel DNA-binding Domain of Helicase-like Transcription Factor (HLTF) and Its Functional Implication in DNA Damage Tolerance.
Hishiki A, Hara K, Ikegaya Y, Yokoyama H, Shimizu T, Sato M, Hashimoto H
J Biol Chem 290 13215-23 (2015)
PMID: 25858588

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helicase-like transcription factor Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 138 amino acids
Theoretical weight: 15.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14527 (Residues: 57-174; Coverage: 12%)
Gene names: HIP116A, HLTF, RNF80, SMARCA3, SNF2L3, ZBU1
Sequence domains: HIRAN domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P41212
Unit cell:
a: 129.441Å b: 129.441Å c: 143.972Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.231
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Replication Fork Reversal: Players and Guardians.
Quinet et al. (2017)
15 more

5 mentions without citation

Control of DNA Damage Bypass by Ubiquitylation of PCNA.
Ripley et al. (2020)
4 more