› 4xzu

X-ray diffraction
2.61Å resolution

Crystal Structure of the Human Factor VIII C2 Domain in Complex with Murine 3E6 Inhibitory Antibody

Released:
DOI: 10.2210/pdb4xzu/pdb
PDB entry 4xzu coloured by chain, front view.
PDB entry 4xzu coloured by chain, side view.
PDB entry 4xzu coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the Human Factor VIII C2 Domain in Complex with the 3E6 Inhibitory Antibody.
Wuerth ME, Cragerud RK, Spiegel PC
Sci Rep 5 17216 (2015)
PMID: 26598467

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208209 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
3E6 antibody Fab heavy chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 219 amino acids
Theoretical weight: 23.54 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
3E6 antibody Fab light chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Factor VIIIa light chain Chains: G, M
Molecule details ›
Chains: G, M
Length: 154 amino acids
Theoretical weight: 17.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00451 (Residues: 2193-2346; Coverage: 7%)
Gene names: F8, F8C
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Unit cell:
a: 43.229Å b: 148.492Å c: 188.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.267
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Novel Coagulation Factor VIII Gene Therapy in a Mouse Model of Hemophilia A by Lipid-Coated Fe3O4 Nanoparticles.
Kao et al. (2021)
1 more

1 mention without citation

Structure of the Human Factor VIII C2 Domain in Complex with the 3E6 Inhibitory Antibody.
Wuerth et al. (2015)