› 4y05

X-ray diffraction
2.59Å resolution

KIF2C short Loop2 construct

Released:
DOI: 10.2210/pdb4y05/pdb
PDB entry 4y05 coloured by chain, front view.
PDB entry 4y05 coloured by chain, side view.
PDB entry 4y05 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
New Insights into the Coupling between Microtubule Depolymerization and ATP Hydrolysis by Kinesin-13 Protein Kif2C.
Wang W, Shen T, Guerois R, Zhang F, Kuerban H, Lv Y, Gigant B, Knossow M, Wang C
J Biol Chem 290 18721-31 (2015)
PMID: 26055718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF2C Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 43.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99661 (Residues: 216-599; Coverage: 52%)
Gene names: KIF2C, KNSL6
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 83.889Å b: 83.889Å c: 147.872Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Review: Mechanochemistry of the kinesin-1 ATPase.
Cross RA. (2016)
2 more

4 mentions without citation

Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.
Benoit et al. (2018)
3 more