› 4y5w

X-ray diffraction
3.1Å resolution

Transcription factor-DNA complex

Released:
DOI: 10.2210/pdb4y5w/pdb
PDB entry 4y5w coloured by chain, front view.
PDB entry 4y5w coloured by chain, side view.
PDB entry 4y5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition by STAT6.
Li J, Rodriguez JP, Niu F, Pu M, Wang J, Hung LW, Shao Q, Zhu Y, Ding W, Liu Y, Da Y, Yao Z, Yang J, Zhao Y, Wei GH, Cheng G, Liu ZJ, Ouyang S
Proc Natl Acad Sci U S A 113 13015-13020 (2016)
PMID: 27803324

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118302 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Signal transducer and activator of transcription 6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 549 amino acids
Theoretical weight: 61.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42226 (Residues: 113-658; Coverage: 65%)
Gene name: STAT6
Sequence domains:
DNA (5'-D(P*AP*TP*GP*GP*AP*TP*TP*TP*CP*CP*TP*AP*GP*GP*AP*AP*GP*AP*CP*AP*A)-3') Chains: E, M
Molecule details ›
Chains: E, M
Length: 22 nucleotides
Theoretical weight: 6.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*TP*GP*TP*CP*TP*TP*CP*CP*TP*AP*GP*GP*AP*AP*AP*TP*CP*CP*AP*T)-3') Chains: F, N
Molecule details ›
Chains: F, N
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 4 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 68.298Å b: 94.241Å c: 147.785Å
α: 99.85° β: 101.73° γ: 89.93°
R-values:
R R work R free
0.251 0.25 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Mechanisms and consequences of Jak-STAT signaling in the immune system.
Villarino et al. (2017)
9 more

4 mentions without citation

The JAK-STAT pathway at 30: Much learned, much more to do.
Philips et al. (2022)
3 more