› 4yce

X-ray diffraction
1.95Å resolution

Crystal structure of the R111K:Y134F:T54V:R132Q:P39Q:R59Y mutant of human cellular retinoic acid binding proteinii with retinal at 1.95 angstrom- visible light irradiated crystal for 1 hour - 2nd cycle

Released:
DOI: 10.2210/pdb4yce/pdb
PDB entry 4yce coloured by chain, front view.
PDB entry 4yce coloured by chain, side view.
PDB entry 4yce coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
Nosrati M, Berbasova T, Vasileiou C, Borhan B, Geiger JH
J Am Chem Soc 138 8802-8 (2016)
PMID: 27310917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular retinoic acid-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 15.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29373 (Residues: 2-138; Coverage: 99%)
Gene name: CRABP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3121
Unit cell:
a: 58.599Å b: 58.599Å c: 99.854Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

11 citation in other articles

Dual Photoisomerization on Distinct Potential Energy Surfaces in a UV-Absorbing Rhodopsin.
Hontani et al. (2020)
10 more

1 mention without citation

A Photoisomerizing Rhodopsin Mimic Observed at Atomic Resolution.
Nosrati et al. (2016)