› 4yea

X-ray diffraction
2.14Å resolution

Crystal structure of cisplatin bound to a human copper chaperone (dimer) - new refinement

Released:
DOI: 10.2210/pdb4yea/pdb
PDB entry 4yea coloured by chain, front view.
PDB entry 4yea coloured by chain, side view.
PDB entry 4yea coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin I, Dauter Z, Jaskolski M, Minor W, Wlodawer A
Acta Crystallogr D Biol Crystallogr 71 1965-79 (2015)
PMID: 26327386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper transport protein ATOX1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00244 (Residues: 2-68; Coverage: 99%)
Gene names: ATOX1, HAH1
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P65
Unit cell:
a: 78.289Å b: 78.289Å c: 54.335Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.197
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

A close look onto structural models and primary ligands of metallo-β-lactamases.
Raczynska et al. (2018)
3 more

2 mentions without citation

Crystallography and chemistry should always go together: a cautionary tale of protein complexes with cisplatin and carboplatin.
Shabalin et al. (2015)
1 more