› 4yhj

X-ray diffraction
2.6Å resolution

Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4 (GRK4)

Released:
DOI: 10.2210/pdb4yhj/pdb
PDB entry 4yhj coloured by chain, front view.
PDB entry 4yhj coloured by chain, side view.
PDB entry 4yhj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4.
Allen SJ, Parthasarathy G, Darke PL, Diehl RE, Ford RE, Hall DL, Johnson SA, Reid JC, Rickert KW, Shipman JM, Soisson SM, Zuck P, Munshi SK, Lumb KJ
J Biol Chem 290 20360-73 (2015)
PMID: 26134571

Function and Biology Details

Reaction catalysed: 
ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-coupled receptor kinase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 67.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32298 (Residues: 1-578; Coverage: 100%)
Gene names: GPRK2L, GPRK4, GRK4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AN2 2 x AN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P3121
Unit cell:
a: 104.838Å b: 104.838Å c: 221.838Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.248
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

G protein-coupled receptor signaling: transducers and effectors.
Jiang et al. (2022)
4 more

2 mentions without citation

Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4.
Allen et al. (2015)
1 more