› 4yj4

X-ray diffraction
2.1Å resolution

Crystal structure of Bcl-xL in complex with the BIM BH3 domain containing Ile155-to-Arg and Glu158-to-phosphoserine mutations

Released:
DOI: 10.2210/pdb4yj4/pdb
PDB entry 4yj4 coloured by chain, front view.
PDB entry 4yj4 coloured by chain, side view.
PDB entry 4yj4 coloured by chain, top view.
Source organisms:
Primary publication:
Conversion of cell-survival activity of Akt into apoptotic death of cancer cells by two mutations on the BIM BH3 domain.
Kim JS, Ku B, Woo TG, Oh AY, Jung YS, Soh YM, Yeom JH, Lee K, Park BJ, Oh BH, Ha NC
Cell Death Dis 6 e1804 (2015)
PMID: 26136077

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128967 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64373 (Residues: 1-196; Coverage: 84%)
Gene names: Bcl2l, Bcl2l1, Bclx
Sequence domains:
Structure domains: Blc2-like
Bcl-2-like protein 11 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43521 (Residues: 146-165; Coverage: 10%)
Gene names: BCL2L11, BIM
Sequence domains: Bcl-x interacting, BH3 domain

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand CL 2 x CL
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 72.926Å b: 72.926Å c: 75.458Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 0.211
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Structural Biology of Bcl-xL.
Lee et al. (2019)
2 other articles