› 4yo2

X-ray diffraction
3.07Å resolution

Structure of E2F8, an atypical member of E2F family of transcription factors

Released:
DOI: 10.2210/pdb4yo2/pdb
PDB entry 4yo2 coloured by chain, front view.
PDB entry 4yo2 coloured by chain, side view.
PDB entry 4yo2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the DNA-binding specificity of E2F family transcription factors.
Morgunova E, Yin Y, Jolma A, Dave K, Schmierer B, Popov A, Eremina N, Nilsson L, Taipale J
Nat Commun 6 10050 (2015)
PMID: 26632596

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106053 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor E2F8 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AVK6 (Residues: 110-341; Coverage: 27%)
Gene name: E2F8
Sequence domains: E2F/DP family winged-helix DNA-binding domain
DNA (5'-D(P*TP*TP*TP*TP*CP*CP*CP*GP*CP*CP*AP*AP*AP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*TP*TP*TP*TP*GP*GP*CP*GP*GP*GP*AP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 98.83Å b: 98.83Å c: 121.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.272 0.272 0.297
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Structural perspective of cooperative transcription factor binding.
Morgunova et al. (2017)
2 more

2 mentions without citation

Detecting and interpreting DNA methylation marks.
Ren et al. (2018)
1 more