› 4ytl

X-ray diffraction
1.6Å resolution

Structure of the KOW2-KOW3 Domain of Transcription Elongation Factor Spt5.

Released:
DOI: 10.2210/pdb4ytl/pdb
PDB entry 4ytl coloured by chain, front view.
PDB entry 4ytl coloured by chain, side view.
PDB entry 4ytl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structures and Functions of the Multiple KOW Domains of Transcription Elongation Factor Spt5.
Meyer PA, Li S, Zhang M, Yamada K, Takagi Y, Hartzog GA, Fu J
Mol Cell Biol 35 3354-69 (2015)
PMID: 26217010

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151047 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor SPT5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27692 (Residues: 534-632; Coverage: 9%)
Gene names: SPT5, YM9571.08, YML010W
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 44.253Å b: 54.243Å c: 95.153Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.203
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Transcription Regulation in Archaea.
Gehring et al. (2016)
2 more

3 mentions without citation

NusG, an Ancient Yet Rapidly Evolving Transcription Factor.
Wang et al. (2020)
2 more