› 4yub

X-ray diffraction
2.9Å resolution

Crystal structure of human Nicotinic Acid Phosphoribosyltransferase

Released:
DOI: 10.2210/pdb4yub/pdb
PDB entry 4yub coloured by chain, front view.
PDB entry 4yub coloured by chain, side view.
PDB entry 4yub coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human nicotinic acid phosphoribosyltransferase.
Marletta AS, Massarotti A, Orsomando G, Magni G, Rizzi M, Garavaglia S
FEBS Open Bio 5 419-28 (2015)
PMID: 26042198

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 538 amino acids
Theoretical weight: 57.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6XQN6 (Residues: 1-538; Coverage: 100%)
Gene names: FHIP, NAPRT, NAPRT1
Sequence domains:
Structure domains: nicotinate phosphoribosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 88.018Å b: 101.091Å c: 125.144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.243
Expression system: Escherichia coli

Quick links

Citations

8 review citations

NAD+ metabolism: pathophysiologic mechanisms and therapeutic potential.
Xie et al. (2020)
7 more

6 mentions without citation

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation.
Managò et al. (2019)
5 more