› 4yyx

X-ray diffraction
1.79Å resolution

Crystal structure of the ZO-1 PDZ1 domain in complex with the 7-mer Claudin2 C-terminal tail

Released:
DOI: 10.2210/pdb4yyx/pdb
PDB entry 4yyx coloured by chain, front view.
PDB entry 4yyx coloured by chain, side view.
PDB entry 4yyx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of a Key Factor Regulating the Affinity between the Zonula Occludens First PDZ Domain and Claudins.
Nomme J, Antanasijevic A, Caffrey M, Van Itallie CM, Anderson JM, Fanning AS, Lavie A
J Biol Chem 290 16595-606 (2015)
PMID: 26023235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Claudin-2; Tight junction protein ZO-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q07157 (Residues: 18-110; Coverage: 5%)
  • Canonical: P57739 (Residues: 224-230; Coverage: 3%)
Gene names: CLDN2, PSEC0059, SP82, TJP1, UNQ705/PRO1356, ZO1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 41.351Å b: 72.17Å c: 76.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.254
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Structure, Function, and Regulation of the Blood-Brain Barrier Tight Junction in Central Nervous System Disorders.
Lochhead et al. (2020)
8 more

2 mentions without citation

Structural Basis of a Key Factor Regulating the Affinity between the Zonula Occludens First PDZ Domain and Claudins.
Nomme et al. (2015)
1 more