› 4z1u

X-ray diffraction
2.05Å resolution

MIF in complex with 1-(4-methylphenyl)-3-phenylprop-2-yn-1-one

Released:
DOI: 10.2210/pdb4z1u/pdb
PDB entry 4z1u coloured by chain, front view.
PDB entry 4z1u coloured by chain, side view.
PDB entry 4z1u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors.
Singh AK, Pantouris G, Borosch S, Rojanasthien S, Cho TY
J Cell Mol Med 21 142-153 (2017)
PMID: 27619729

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 114 amino acids
Theoretical weight: 12.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

2 bound ligands:
Ligand SO4 15 x SO4
Ligand GOL 8 x GOL
1 modified residue:
Ligand 4N7 6 x 4N7

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 94.97Å b: 105.96Å c: 106.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Function, Regulation and Biological Roles of PI3Kγ Variants.
Nürnberg et al. (2019)
1 more

2 mentions without citation

Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors.
Singh et al. (2017)
1 more