› 4z4k

X-ray diffraction
2.8Å resolution

Crystal structure of GFP-TAX1BP1 UBZ1+2 domain fusion protein

Released:
DOI: 10.2210/pdb4z4k/pdb
PDB entry 4z4k coloured by chain, front view.
PDB entry 4z4k coloured by chain, side view.
PDB entry 4z4k coloured by chain, top view.
Source organisms:
Primary publication:
A novel mode of ubiquitin recognition by the ubiquitin-binding zinc finger domain of WRNIP1.
Suzuki N, Rohaim A, Kato R, Dikic I, Wakatsuki S, Kawasaki M
FEBS J 283 2004-17 (2016)
PMID: 27062441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Tax1-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.25 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
  • Canonical: Q86VP1 (Residues: 725-781; Coverage: 7%)
Gene names: GFP, PRO0105, T6BP, TAX1BP1
Sequence domains:
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 56.885Å b: 83.334Å c: 128.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.206 0.28
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Autophagy in Neurodegenerative Diseases: A Hunter for Aggregates.
Park et al. (2020)
2 more

2 mentions without citation

Cas6 processes tight and relaxed repeat RNA via multiple mechanisms: A hypothesis.
Sefcikova et al. (2017)
1 more