› 4z8y

X-ray diffraction
1.9Å resolution

Crystal structure of Rab GTPase Sec4p mutant - S29V

Released:
DOI: 10.2210/pdb4z8y/pdb
PDB entry 4z8y coloured by chain, front view.
PDB entry 4z8y coloured by chain, side view.
PDB entry 4z8y coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
New insights into the molecular mechanism of the Rab GTPase Sec4p activation.
Rinaldi FC, Packer M, Collins R
BMC Struct Biol 15 14 (2015)
PMID: 26263895

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein SEC4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07560 (Residues: 19-187; Coverage: 79%)
Gene names: SEC4, SRO6, YFL005W
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 31.72Å b: 75.45Å c: 66.23Å
α: 90° β: 91.58° γ: 90°
R-values:
R R work R free
0.239 0.236 0.298
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
5 other articles

1 mention without citation

New insights into the molecular mechanism of the Rab GTPase Sec4p activation.
Rinaldi et al. (2015)