4zbj

X-ray diffraction
2.25Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152307 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone chaperone ASF1 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.66 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 2-169; Coverage: 60%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like
Histone H3.3 Chain: B
Molecule details ›
Chain: B
Length: 77 amino acids
Theoretical weight: 8.81 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PI79 (Residues: 62-136; Coverage: 55%)
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A
Histone H4 Chain: C
Molecule details ›
Chain: C
Length: 84 amino acids
Theoretical weight: 9.54 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 21-103; Coverage: 81%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Ubinuclein-1 Chain: D
Molecule details ›
Chain: D
Length: 28 amino acids
Theoretical weight: 3.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9NPG3 (Residues: 122-148; Coverage: 2%)
Gene name: UBN1
Sequence domains: HPC2 and ubinuclein domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P3221
Unit cell:
a: 90.024Å b: 90.024Å c: 120.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.235
Expression systems:
  • Escherichia coli
  • Not provided