› 4zcr

X-ray diffraction
1.8Å resolution

Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with phosphocholine

Released:
DOI: 10.2210/pdb4zcr/pdb
PDB entry 4zcr coloured by chain, front view.
PDB entry 4zcr coloured by chain, side view.
PDB entry 4zcr coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis.
Guca E, Nagy GN, Hajdú F, Marton L, Izrael R, Hoh F, Yang Y, Vial H, Vértessy BG, Guichou JF, Cerdan R
Sci Rep 8 11215 (2018)
PMID: 30046154

Function and Biology Details

Reaction catalysed: 
CTP + phosphocholine = diphosphate + CDP-choline
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
choline-phosphate cytidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.81 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IEE9 (Residues: 581-775; Coverage: 20%)
Gene name: PF3D7_1316600
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand PC 1 x PC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 50.62Å b: 69.57Å c: 117.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.232
Expression system: Escherichia coli

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Citations

1 review citation

Malaria therapeutics: are we close enough?
Tripathi et al. (2023)
3 other articles

2 mentions without citation

Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis.
Guca et al. (2018)
1 more