› 4zcs

X-ray diffraction
2.45Å resolution

Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CDP-choline

Released:
DOI: 10.2210/pdb4zcs/pdb
PDB entry 4zcs coloured by chain, front view.
PDB entry 4zcs coloured by chain, side view.
PDB entry 4zcs coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis.
Guca E, Nagy GN, Hajdú F, Marton L, Izrael R, Hoh F, Yang Y, Vial H, Vértessy BG, Guichou JF, Cerdan R
Sci Rep 8 11215 (2018)
PMID: 30046154

Function and Biology Details

Reaction catalysed: 
CTP + phosphocholine = diphosphate + CDP-choline
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
choline-phosphate cytidylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 180 amino acids
Theoretical weight: 20.81 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IEE9 (Residues: 581-775; Coverage: 20%)
Gene name: PF3D7_1316600
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand CDC 6 x CDC
Ligand P33 2 x P33
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 114.631Å b: 149.132Å c: 175.598Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.234
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Malaria therapeutics: are we close enough?
Tripathi et al. (2023)
3 other articles

2 mentions without citation

Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis.
Guca et al. (2018)
1 more