› 4zcw

X-ray diffraction
1.99Å resolution

Structure of Human Enolase 2 in complex with SF2312

Released:
DOI: 10.2210/pdb4zcw/pdb
PDB entry 4zcw coloured by chain, front view.
PDB entry 4zcw coloured by chain, side view.
PDB entry 4zcw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
SF2312 is a natural phosphonate inhibitor of enolase.
Leonard PG, Satani N, Maxwell D, Lin YH, Hammoudi N, Peng Z, Pisaneschi F, Link TM, Lee GR, Sun D, Prasad BAB, Di Francesco ME, Czako B, Asara JM, Wang YA, Bornmann W, DePinho RA, Muller FL
Nat Chem Biol 12 1053-1058 (2016)
PMID: 27723749

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-enolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 48.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P09104 (Residues: 1-434; Coverage: 100%)
Gene name: ENO2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 4NG 2 x 4NG
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 68.3Å b: 109.46Å c: 117.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

Targeting Metabolism for Cancer Therapy.
Luengo et al. (2017)
11 more

5 mentions without citation

SF2312 is a natural phosphonate inhibitor of enolase.
Leonard et al. (2016)
4 more