› 4zii

X-ray diffraction
2.19Å resolution

Crystal Structure of core/latch dimer of BaxI66A in complex with BidBH3

Released:
DOI: 10.2210/pdb4zii/pdb
PDB entry 4zii coloured by chain, front view.
PDB entry 4zii coloured by chain, side view.
PDB entry 4zii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of Bax bound to the BH3 peptide of Bim identifies important contacts for interaction.
Robin AY, Krishna Kumar K, Westphal D, Wardak AZ, Thompson GV, Dewson G, Colman PM, Czabotar PE
Cell Death Dis 6 e1809 (2015)
PMID: 26158515

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157371 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptosis regulator BAX Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 18.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07812 (Residues: 1-170; Coverage: 89%)
Gene names: BAX, BCL2L4
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
BH3-interacting domain death agonist p11 Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 3.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P55957 (Residues: 76-109; Coverage: 17%)
Gene name: BID

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 103.801Å b: 103.801Å c: 38.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.175 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

BCL-2 family proteins: changing partners in the dance towards death.
Kale et al. (2018)
14 more

3 mentions without citation

Crystal structure of Bax bound to the BH3 peptide of Bim identifies important contacts for interaction.
Robin et al. (2015)
2 more