› 4zjs

X-ray diffraction
2.23Å resolution

Crystal structure of a chimeric acetylcholine binding protein from Aplysia Californica (Ac-AChBP) containing the main immunogenic region (MIR) from the human alpha 1 subunit of the muscle nicotinic acetylcholine receptor in complex with anatoxin-A.

Released:
DOI: 10.2210/pdb4zjs/pdb
PDB entry 4zjs coloured by chain, front view.
PDB entry 4zjs coloured by chain, side view.
PDB entry 4zjs coloured by chain, top view.
Source organisms:
Primary publication:
Main immunogenic region structure promotes binding of conformation-dependent myasthenia gravis autoantibodies, nicotinic acetylcholine receptor conformation maturation, and agonist sensitivity.
Luo J, Taylor P, Losen M, de Baets MH, Shelton GD, Lindstrom J
J Neurosci 29 13898-908 (2009)
PMID: 19890000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine receptor subunit alpha; Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 230 amino acids
Theoretical weight: 26.31 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P02708 (Residues: 21-52, 81-101; Coverage: 12%)
  • Canonical: Q8WSF8 (Residues: 50-77, 99-236; Coverage: 77%)
Gene names: ACHRA, CHNRA, CHRNA1
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

1 bound ligand:
Ligand 4P0 3 x 4P0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I23
Unit cell:
a: 207.604Å b: 207.604Å c: 207.604Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.228
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Mechanisms of Autoantibody-Induced Pathology.
Ludwig et al. (2017)
14 more

1 mention without citation

Delineating the activity of the potent nicotinic acetylcholine receptor agonists (+)-anatoxin-a and (-)-hosieine-A.
Parker et al. (2022)