› 4zk4

X-ray diffraction
1.9Å resolution

Crystal structure of a chimeric acetylcholine binding protein from Aplysia californica (Ac-AChBP) containing loop C from the human alpha 3 nicotinic acetylcholine receptor in complex with 7-(5-isopropoxy-pyridin-3-yl)-1-methyl-1,7-diaza-spiro[4.4]nonane

Released:
DOI: 10.2210/pdb4zk4/pdb
PDB entry 4zk4 coloured by chain, front view.
PDB entry 4zk4 coloured by chain, side view.
PDB entry 4zk4 coloured by chain, top view.
Source organisms:
Entry authors: Bobango J, Wu J, Talley TT

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal acetylcholine receptor subunit alpha-3; Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 229 amino acids
Theoretical weight: 26.2 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WSF8 (Residues: 18-71, 73-198, 215-236; Coverage: 92%)
  • Canonical: P32297 (Residues: 86-86, 215-230; Coverage: 4%)
Gene names: CHRNA3, NACHRA3
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand SO4 5 x SO4
Ligand PG4 1 x PG4
Ligand TII 2 x TII
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 70.662Å b: 78.205Å c: 120.465Å
α: 90° β: 94.97° γ: 90°
R-values:
R R work R free
0.183 0.181 0.212
Expression system: Homo sapiens

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