› 4zso

X-ray diffraction
2.5Å resolution

Crystal structure of a complex between B7-H6, a tumor cell ligand for natural cytotoxicity receptor NKp30, and an inhibitory antibody

Released:
DOI: 10.2210/pdb4zso/pdb
PDB entry 4zso coloured by chain, front view.
PDB entry 4zso coloured by chain, side view.
PDB entry 4zso coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Xu X, Li Y, Mariuzza RA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212342 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (6 distinct):
Antibody Light Chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Antibody Heavy Chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 219 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Natural cytotoxicity triggering receptor 3 ligand 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 253 amino acids
Theoretical weight: 28.32 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q68D85 (Residues: 25-262; Coverage: 55%)
Gene names: B7H6, NCR3LG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 2 x SO4
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 89.634Å b: 138.029Å c: 171.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • unidentified baculovirus

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