› 4zto

X-ray diffraction
2.3Å resolution

Fab/epitope complex structure of rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region

Released:
DOI: 10.2210/pdb4zto/pdb
PDB entry 4zto coloured by chain, front view.
PDB entry 4zto coloured by chain, side view.
PDB entry 4zto coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis of a novel rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region critical for receptor and co-receptor binding.
Pan R, Chen Y, Vaine M, Hu G, Wang S, Lu S, Kong XP
Emerg Microbes Infect 4 e44 (2015)
PMID: 26251831

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229837 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RABBIT MONOCLONAL ANTIBODY R53 FAB LIGHT CHAIN Chains: L, M
Molecule details ›
Chains: L, M
Length: 216 amino acids
Theoretical weight: 23.02 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
RABBIT MONOCLONAL ANTIBODY R53 FAB HEAVY CHAIN Chains: H, I
Molecule details ›
Chains: H, I
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Surface protein gp120 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 15 amino acids
Theoretical weight: 1.66 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 430-444; Coverage: 2%)
Gene name: env

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 72.283Å b: 84.831Å c: 167.237Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.167 0.222
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

3 review citations

From rabbit antibody repertoires to rabbit monoclonal antibodies.
Weber et al. (2017)
2 more

4 mentions without citation

A catalogue of biochemically diverse CRISPR-Cas9 orthologs.
Gasiunas et al. (2020)
3 more