› 4zuk

X-ray diffraction
2Å resolution

Structure ALDH7A1 complexed with NAD+

Released:
DOI: 10.2210/pdb4zuk/pdb
PDB entry 4zuk coloured by chain, front view.
PDB entry 4zuk coloured by chain, side view.
PDB entry 4zuk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Luo M, Tanner JJ
Biochemistry 54 5513-22 (2015)
PMID: 26260980

Function and Biology Details

Reactions catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
(1a) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H(2)O (spontaneous)
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-aminoadipic semialdehyde dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 513 amino acids
Theoretical weight: 55.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49419 (Residues: 29-539; Coverage: 95%)
Gene names: ALDH7A1, ATQ1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
1 bound ligand:
Ligand PG4 8 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 155.666Å b: 161.579Å c: 158.932Å
α: 90° β: 94.76° γ: 90°
R-values:
R R work R free
0.173 0.171 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Molecular Mechanisms of Bacterial Bioluminescence.
Brodl et al. (2018)
3 more

6 mentions without citation

Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Luo et al. (2015)
5 more