› 4zwe

X-ray diffraction
2.81Å resolution

Crystal structure of the dGTP-bound catalytic core of SAMHD1 T592V mutant

Released:
DOI: 10.2210/pdb4zwe/pdb
PDB entry 4zwe coloured by chain, front view.
PDB entry 4zwe coloured by chain, side view.
PDB entry 4zwe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Impaired dNTPase activity of SAMHD1 by phosphomimetic mutation of Thr-592.
Tang C, Ji X, Wu L, Xiong Y
J Biol Chem 290 26352-9 (2015)
PMID: 26294762

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 514 amino acids
Theoretical weight: 59.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 113-626; Coverage: 82%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand DGT 12 x DGT
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 89.083Å b: 146.091Å c: 98.463Å
α: 90° β: 115.03° γ: 90°
R-values:
R R work R free
0.21 0.208 0.248
Expression system: Escherichia coli

Quick links

Citations

14 review citations

SAMHD1 Functions and Human Diseases.
Coggins et al. (2020)
13 more

2 mentions without citation

Impaired dNTPase activity of SAMHD1 by phosphomimetic mutation of Thr-592.
Tang et al. (2015)
1 more