› 4a0x

X-ray diffraction
2.4Å resolution

Structure of the global transcription regulator FapR from Staphylococcus aureus

Released:
DOI: 10.2210/pdb4a0x/pdb
PDB entry 4a0x coloured by chain, front view.
PDB entry 4a0x coloured by chain, side view.
PDB entry 4a0x coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis for feed-forward transcriptional regulation of membrane lipid homeostasis in Staphylococcus aureus.
Albanesi D, Reh G, Guerin ME, Schaeffer F, Debarbouille M, Buschiazzo A, Schujman GE, de Mendoza D, Alzari PM
PLoS Pathog 9 e1003108 (2013)
PMID: 23300457

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor FapR Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22.03 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2FZ56 (Residues: 1-190; Coverage: 100%)
Gene names: SAOUHSC_01196, fapR
Sequence domains: Thioesterase superfamily
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 5 x ZN
Ligand ACT 2 x ACT
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R32
Unit cell:
a: 116.58Å b: 116.58Å c: 123.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.246
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Bacterial lipids: metabolism and membrane homeostasis.
Parsons et al. (2013)
8 more

1 mention without citation

Structural basis for feed-forward transcriptional regulation of membrane lipid homeostasis in Staphylococcus aureus.
Albanesi et al. (2013)