› 4a3q

X-ray diffraction
2.15Å resolution

The 2.15 Angstrom resolution crystal structure of Staphylococcus aureus alanine racemase

Released:
DOI: 10.2210/pdb4a3q/pdb
PDB entry 4a3q coloured by chain, front view.
PDB entry 4a3q coloured by chain, side view.
PDB entry 4a3q coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50).
Scaletti ER, Luckner SR, Krause KL
Acta Crystallogr D Biol Crystallogr 68 82-92 (2012)
PMID: 22194336

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 42.86 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63479 (Residues: 1-382; Coverage: 100%)
Gene names: SAV2070, alr, alr1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand SO4 13 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 65.14Å b: 113.94Å c: 125.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.236
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Assembly of Peptidoglycan Fragments-A Synthetic Challenge.
Queda et al. (2020)
10 other articles

6 mentions without citation

Mining the cellular inventory of pyridoxal phosphate-dependent enzymes with functionalized cofactor mimics.
Hoegl et al. (2018)
5 more