› 4a4x

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Nek2 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 32.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51955 (Residues: 1-271; Coverage: 61%)
Gene names: NEK2, NEK2A, NLK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand JUP 1 x JUP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 101.223Å b: 56.909Å c: 74.408Å
α: 90° β: 127.12° γ: 90°
R-values:
R R work R free
0.206 0.204 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

On the molecular mechanisms of mitotic kinase activation.
Bayliss et al. (2012)
8 more

2 mentions without citation

Cell cycle regulation by the NEK family of protein kinases.
Fry et al. (2012)
1 more