› 4a5p

X-ray diffraction
3.15Å resolution

Structure of the Shigella flexneri MxiA protein

Released:
DOI: 10.2210/pdb4a5p/pdb
PDB entry 4a5p coloured by chain, front view.
PDB entry 4a5p coloured by chain, side view.
PDB entry 4a5p coloured by chain, top view.
Source organism: Shigella flexneri 5a
Primary publication:
Architecture of the major component of the type III secretion system export apparatus.
Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM
Nat Struct Mol Biol 20 99-104 (2013)
PMID: 23222644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo nonamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein MxiA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 383 amino acids
Theoretical weight: 44.08 KDa
Source organism: Shigella flexneri 5a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A1I5 (Residues: 318-686; Coverage: 54%)
Gene names: CP0147, mxiA, virH
Sequence domains: FHIPEP family
Structure domains: FHIPEP family, domain 1

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
1 modified residue:
Ligand MLY 39 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P63
Unit cell:
a: 160.61Å b: 160.61Å c: 100.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.231 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
37 more

15 mentions without citation

Building a secreting nanomachine: a structural overview of the T3SS.
Abrusci et al. (2014)
14 more