› 4a5x

X-ray diffraction
1.91Å resolution

Structures of MITD1

Released:
DOI: 10.2210/pdb4a5x/pdb
PDB entry 4a5x coloured by chain, front view.
PDB entry 4a5x coloured by chain, side view.
PDB entry 4a5x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.
Hadders MA, Agromayor M, Obita T, Perisic O, Caballe A, Kloc M, Lamers MH, Williams RL, Martin-Serrano J
Proc Natl Acad Sci U S A 109 17424-9 (2012)
PMID: 23045692

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186732 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MIT domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 10.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WV92 (Residues: 9-85; Coverage: 31%)
Gene name: MITD1
Sequence domains: MIT (microtubule interacting and transport) domain
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
Charged multivesicular body protein 1a Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HD42 (Residues: 184-196; Coverage: 7%)
Gene names: CHMP1, CHMP1A, KIAA0047, PCOLN3, PRSM1

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand P15 1 x P15
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 67.07Å b: 74.16Å c: 89.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

The many functions of ESCRTs.
Vietri et al. (2020)
13 more

3 mentions without citation

Mechanistic Insights into Side Effects of Troglitazone and Rosiglitazone Using a Novel Inverse Molecular Docking Protocol.
Kores et al. (2021)
2 more