› 4a7j

X-ray diffraction
1.9Å resolution

Symmetric Dimethylation of H3 Arginine 2 is a Novel Histone Mark that Supports Euchromatin Maintenance

Released:
DOI: 10.2210/pdb4a7j/pdb
PDB entry 4a7j coloured by chain, front view.
PDB entry 4a7j coloured by chain, side view.
PDB entry 4a7j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Symmetric dimethylation of H3R2 is a newly identified histone mark that supports euchromatin maintenance.
Migliori V, Müller J, Phalke S, Low D, Bezzi M, Mok WC, Sahu SK, Gunaratne J, Capasso P, Bassi C, Cecatiello V, De Marco A, Blackstock W, Kuznetsov V, Amati B, Mapelli M, Guccione E
Nat Struct Mol Biol 19 136-44 (2012)
PMID: 22231400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158475 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 34.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61964 (Residues: 21-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.72 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 1-16; Coverage: 12%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 2MR 1 x 2MR

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 52.6Å b: 46.8Å c: 65Å
α: 90° β: 104.33° γ: 90°
R-values:
R R work R free
0.184 0.184 0.222
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

69 review citations

Protein arginine methyltransferases and cancer.
Yang et al. (2013)
68 more

7 mentions without citation

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
6 more