› 4a8x

X-ray diffraction
1.9Å resolution

Structure of the core ASAP complex

Released:
DOI: 10.2210/pdb4a8x/pdb
PDB entry 4a8x coloured by chain, front view.
PDB entry 4a8x coloured by chain, side view.
PDB entry 4a8x coloured by chain, top view.
Source organisms:
Primary publication:
The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.
Murachelli AG, Ebert J, Basquin C, Le Hir H, Conti E
Nat Struct Mol Biol 19 378-86 (2012)
PMID: 22388736

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-129560 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RNA-binding protein with serine-rich domain 1 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15287 (Residues: 159-244; Coverage: 28%)
Gene names: LDC2, RNPS1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
LD23757p Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.71 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9VJ12 (Residues: 648-687; Coverage: 5%)
Gene names: Acn, Ba, CG10437, CG10473, Dmel\CG10473, Dmel_CG10473, acn, dAcn, dacn, hkl, l(2)37Ba
Sequence domains: RNSP1-SAP18 binding (RSB) motif
Histone deacetylase complex subunit SAP18 Chain: C
Molecule details ›
Chain: C
Length: 130 amino acids
Theoretical weight: 14.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55128 (Residues: 14-143; Coverage: 85%)
Gene name: Sap18
Sequence domains: Sin3 associated polypeptide p18 (SAP18)
Structure domains: ASAP complex, SAP18 subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 38.868Å b: 73.331Å c: 50.754Å
α: 90° β: 110.99° γ: 90°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The exon junction complex as a node of post-transcriptional networks.
Le Hir et al. (2016)
7 more

6 mentions without citation

Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.
Deka et al. (2017)
5 more