› 4aab

X-ray diffraction
2.5Å resolution

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Released:
DOI: 10.2210/pdb4aab/pdb
PDB entry 4aab coloured by chain, front view.
PDB entry 4aab coloured by chain, side view.
PDB entry 4aab coloured by chain, top view.
Source organisms:
Primary publication:
Non-specific protein-DNA interactions control I-CreI target binding and cleavage.
Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Epinat JC, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G
Nucleic Acids Res 40 6936-45 (2012)
PMID: 22495931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117207 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
14MER DNA 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: synthetic construct
Expression system: Not provided
10MER DNA 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 10 nucleotides
Theoretical weight: 3.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand PGO 5 x PGO
Ligand MG 3 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS
Spacegroup: P21212
Unit cell:
a: 171.97Å b: 71.1Å c: 46.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.225
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Applications of Alternative Nucleases in the Age of CRISPR/Cas9.
Guha et al. (2017)
15 other articles

3 mentions without citation

Non-specific protein-DNA interactions control I-CreI target binding and cleavage.
Molina et al. (2012)
2 more