› 4aae

X-ray diffraction
2.6Å resolution

Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3')

Released:
DOI: 10.2210/pdb4aae/pdb
PDB entry 4aae coloured by chain, front view.
PDB entry 4aae coloured by chain, side view.
PDB entry 4aae coloured by chain, top view.
Source organisms:
Primary publication:
Non-specific protein-DNA interactions control I-CreI target binding and cleavage.
Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Epinat JC, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G
Nucleic Acids Res 40 6936-45 (2012)
PMID: 22495931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117209 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.6 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-154; Coverage: 94%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
24MER DNA Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.39 KDa
Source organism: synthetic construct
Expression system: Not provided
24MER DNA Chain: G
Molecule details ›
Chain: G
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS
Unit cell:
a: 49.15Å b: 70.34Å c: 172.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.175 0.242
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Applications of Alternative Nucleases in the Age of CRISPR/Cas9.
Guha et al. (2017)
15 other articles

1 mention without citation

Non-specific protein-DNA interactions control I-CreI target binding and cleavage.
Molina et al. (2012)