› 4apc

X-ray diffraction
2.1Å resolution

Crystal Structure of Human NIMA-related Kinase 1 (NEK1)

Released:
DOI: 10.2210/pdb4apc/pdb
PDB entry 4apc coloured by chain, front view.
PDB entry 4apc coloured by chain, side view.
PDB entry 4apc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NEK1 kinase domain structure and its dynamic protein interactome after exposure to Cisplatin.
Melo-Hanchuk TD, Slepicka PF, Meirelles GV, Basei FL, Lovato DV, Granato DC, Pauletti BA, Domingues RR, Leme AFP, Pelegrini AL, Lenz G, Knapp S, Elkins JM, Kobarg J
Sci Rep 7 5445 (2017)
PMID: 28710492

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Nek1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 39.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96PY6 (Residues: 1-328; Coverage: 26%)
Gene names: KIAA1901, NEK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 7 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2221
Unit cell:
a: 89.91Å b: 92.81Å c: 163.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Mitotic Regulation by NEK Kinase Networks.
Fry et al. (2017)
7 more

5 mentions without citation

On Broken Ne(c)ks and Broken DNA: The Role of Human NEKs in the DNA Damage Response.
Pavan et al. (2021)
4 more