› 4atz

X-ray diffraction
1.95Å resolution

Ad5 knob in complex with a designed ankyrin repeat protein

Released:
DOI: 10.2210/pdb4atz/pdb
PDB entry 4atz coloured by chain, front view.
PDB entry 4atz coloured by chain, side view.
PDB entry 4atz coloured by chain, top view.
Source organisms:
Primary publication:
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters.
Dreier B, Honegger A, Hess C, Nagy-Davidescu G, Mittl PR, Grütter MG, Belousova N, Mikheeva G, Krasnykh V, Plückthun A
Proc Natl Acad Sci U S A 110 E869-77 (2013)
PMID: 23431166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145936 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fiber protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 201 amino acids
Theoretical weight: 21.89 KDa
Source organism: Human adenovirus 5
Expression system: Escherichia coli
UniProt:
  • Canonical: P11818 (Residues: 387-581; Coverage: 33%)
Gene name: L5
Sequence domains: Adenoviral fibre protein (knob domain)
Structure domains: Adenovirus pIV-related, attachment domain
DESIGNED ANKYRIN REPEAT PROTEIN Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 154 amino acids
Theoretical weight: 16.47 KDa
Source organism: synthetic construct
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 109.6Å b: 112.1Å c: 129.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.193
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
16 more

2 mentions without citation

Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters.
Dreier et al. (2013)
1 more