› 4awe

X-ray diffraction
1.4Å resolution

The Crystal Structure of Chrysonilia sitophila endo-beta-D-1,4- mannanase

Released:
DOI: 10.2210/pdb4awe/pdb
PDB entry 4awe coloured by chain, front view.
PDB entry 4awe coloured by chain, side view.
PDB entry 4awe coloured by chain, top view.
Source organism: Neurospora sitophila
Primary publication:
Endo-β-D-1,4-mannanase from Chrysonilia sitophila displays a novel loop arrangement for substrate selectivity.
Gonçalves AM, Silva CS, Madeira TI, Coelho R, de Sanctis D, San Romão MV, Bento I
Acta Crystallogr D Biol Crystallogr 68 1468-78 (2012)
PMID: 23090396

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125118 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 43.8 KDa
Source organism: Neurospora sitophila
UniProt:
  • Canonical: I4IY26 (Residues: 1-385; Coverage: 100%)
Gene name: man5
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand TRS 1 x TRS
Ligand ACT 2 x ACT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 57.36Å b: 78.94Å c: 83.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.202

Quick links

Citations

7 citation in other articles

Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus.
de Vries et al. (2017)
6 more

5 mentions without citation

Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides.
Déjean et al. (2020)
4 more