› 4az0

X-ray diffraction
2.17Å resolution

crystal structure of cathepsin a, complexed with 8a.

Released:
DOI: 10.2210/pdb4az0/pdb
PDB entry 4az0 coloured by chain, front view.
PDB entry 4az0 coloured by chain, side view.
PDB entry 4az0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel β-amino acid derivatives as inhibitors of cathepsin A.
Ruf S, Buning C, Schreuder H, Horstick G, Linz W, Olpp T, Pernerstorfer J, Hiss K, Kroll K, Kannt A, Kohlmann M, Linz D, Hübschle T, Rütten H, Wirth K, Schmidt T, Sadowski T
J Med Chem 55 7636-49 (2012)
PMID: 22861813

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid with broad specificity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145364 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Lysosomal protective protein 32 kDa chain Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10619 (Residues: 29-326; Coverage: 66%)
Gene names: CTSA, PPGB
Sequence domains: Serine carboxypeptidase
Structure domains: alpha/beta hydrolase
Lysosomal protective protein 20 kDa chain Chain: B
Molecule details ›
Chain: B
Length: 155 amino acids
Theoretical weight: 18.07 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10619 (Residues: 327-480; Coverage: 34%)
Gene names: CTSA, PPGB
Sequence domains: Serine carboxypeptidase
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand S61 1 x S61
Ligand CD 6 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 90.53Å b: 101.638Å c: 48.179Å
α: 90° β: 101.97° γ: 90°
Expression system: Spodoptera frugiperda

Quick links

Citations

12 citation in other articles

Cathepsin A mediates susceptibility to atrial tachyarrhythmia and impairment of atrial emptying function in Zucker diabetic fatty rats.
Linz et al. (2016)
11 more

2 mentions without citation

Proteolytic activation of human cathepsin A.
Kolli et al. (2014)
1 more