› 4azu

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP

Released:
DOI: 10.2210/pdb4azu/pdb
PDB entry 4azu coloured by chain, front view.
PDB entry 4azu coloured by chain, side view.
PDB entry 4azu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure analysis of oxidized Pseudomonas aeruginosa azurin at pH 5.5 and pH 9.0. A pH-induced conformational transition involves a peptide bond flip.
Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW
J Mol Biol 221 765-72 (1991)
PMID: 1942029

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 4 x CU
Ligand NO3 1 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 57.65Å b: 80.93Å c: 110.17Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

15 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
14 more

46 mentions without citation

Design of functional metalloproteins.
Lu et al. (2009)
45 more