› 4b7y

X-ray diffraction
3.25Å resolution

Crystal structure of the MSL1-MSL2 complex

Released:
DOI: 10.2210/pdb4b7y/pdb
PDB entry 4b7y coloured by chain, front view.
PDB entry 4b7y coloured by chain, side view.
PDB entry 4b7y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila.
Hallacli E, Lipp M, Georgiev P, Spielman C, Cusack S, Akhtar A, Kadlec J
Mol Cell 48 587-600 (2012)
PMID: 23084835

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179332 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Male-specific lethal 1 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 44 amino acids
Theoretical weight: 4.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q68DK7 (Residues: 212-252; Coverage: 7%)
Gene names: MSL1, MSL1L1
Sequence domains: Dimerisation domain of Male-specific-Lethal 1
E3 ubiquitin-protein ligase MSL2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 116 amino acids
Theoretical weight: 13.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HCI7 (Residues: 1-116; Coverage: 20%)
Gene names: KIAA1585, MSL2, MSL2L1, RNF184
Sequence domains: zinc RING finger of MSL2
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 100.99Å b: 100.99Å c: 88.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.244 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Dosage compensation in Drosophila.
Lucchesi et al. (2015)
8 more

1 mention without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)