› 4bc3

X-ray diffraction
1.68Å resolution

Crystal structure of human D-xylulokinase

Released:
DOI: 10.2210/pdb4bc3/pdb
PDB entry 4bc3 coloured by chain, front view.
PDB entry 4bc3 coloured by chain, side view.
PDB entry 4bc3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism.
Bunker RD, Bulloch EM, Dickson JM, Loomes KM, Baker EN
J Biol Chem 288 1643-52 (2013)
PMID: 23179721

Function and Biology Details

Reaction catalysed: 
ATP + D-xylulose = ADP + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylulose kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 538 amino acids
Theoretical weight: 59.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75191 (Residues: 1-536; Coverage: 100%)
Gene name: XYLB
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

1 bound ligand:
Ligand EDO 10 x EDO
1 modified residue:
Ligand MSE 39 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P32
Unit cell:
a: 99.79Å b: 99.79Å c: 157.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Assessing and maximizing data quality in macromolecular crystallography.
Karplus et al. (2015)
1 more

3 mentions without citation

Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism.
Bunker et al. (2013)
2 more