› 4bdw

X-ray diffraction
2.5Å resolution

The structure of the FnI-EGF tandem domain of coagulation factor XII in complex with Holmium

Released:
DOI: 10.2210/pdb4bdw/pdb
PDB entry 4bdw coloured by chain, front view.
PDB entry 4bdw coloured by chain, side view.
PDB entry 4bdw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the FnI-EGF-like tandem domain of coagulation factor XII solved using SIRAS.
Beringer DX, Kroon-Batenburg LM
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 94-102 (2013)
PMID: 23385745

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coagulation factor XIIa heavy chain Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.54 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00748 (Residues: 133-215; Coverage: 14%)
Gene name: F12
Sequence domains:
Structure domains: Laminin

Ligands and Environments

2 bound ligands:
Ligand HO 2 x HO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 96.245Å b: 96.245Å c: 47.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.235
Expression system: Komagataella pastoris

Quick links

Citations

8 citation in other articles

Coagulation factor XII protease domain crystal structure.
Pathak et al. (2015)
7 more

1 mention without citation

The structure of the FnI-EGF-like tandem domain of coagulation factor XII solved using SIRAS.
Beringer et al. (2013)